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KMID : 0370819920070010019
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Yonsei Journal Dental Science
1992 Volume.7 No. 1 p.19 ~ p.33
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SENSITIVITY OF DIFFERENT TESTING METHOD IN THE CYTOTOXICITY OF THE ROOT CANAL SEALERS
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Abstract
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To investigate and compare the cytotoxic sensitivity of the root canal sealers to the different testing method, this study has been done on the 4 kinds of sealer using agar overlay method(measuring the permeability of the cell membrane by vital
stain),
MTT assay(measuring the activity of succinate dehydrogenase in mitochondria) and Neutral red assay(measuring the activity of lysosome) with the L929 cell of the mouse fibroblast. Also all specimens were reacted with the cells immediately, 24
hours
and
72 hours after mixing in order to study the changes of cytotxicity according to setting process.
@ES Results were as follows :
@EN 1. For the agar overlay method, Nogenol and Seqlapex showed moderate toxicity and AH26 and tubli-seal showed mild toxicity and all sealers except Sealapex appears to be less toxic as the time passed after mixing.
2. For the MTT assay, AH26 showed severe, Sealapex and Tubli-seal moderate and Nogenol nontoxic.
3. For the MTT assay, cytotoxicity increased with the lapse of the cultivation time, but AH26 and Sealapex appears to be more toxic as the time passed after mixing.
4. For the neutral red assay, all sealers were more toxic at 6 hours after cultivation than 12hours and the cytotoxicity were decreased with the lapse of the cultivation time regardless of the time sequence after mixing.
As the result of this study, the sensitivity in the cytotoxicity of same root canal sealer did not show the good correlation in the case of agar overlay method and MTT assay. As the neutral red assay has been done to simulate the clinical
situation
and
the more sensitive reactions of all sealers were showed, the neutral red assay is considered to be the adequate testing method of cytotoxicity for the root canal sealers.
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KEYWORD
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